PSEUDOMONAS (CETRIMIDE) AGAR is recommended by the European Pharmacopoeia for the isolation and identification of Pseudomonas strains. The medium promotes the production of fluorescein (pyoverdin), a green-yellow fluorescent pigment that oxidizes to yellow. It is water-soluble and, unlike pyocyanin (blue-green pigment), is not soluble in chloroform. The pigment diffuses throughout the medium and the fluorescent yellow-green color is observed.
Pancreatic digest of gelatin
20.0
Magnesium Chloride
1.4
Dipotassium Sulphate
10.0
Cetrimide
0.3
Agar
15.0
Final pH
7.2 ± 0.2 at 25 °C
Appearance
free-flowing, homogeneous.
Color
very light beige
Storage
10-30 °C, in a dry environment
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